to Severe AcuteRespiratory Syndrome (SARS-CoV), Urbani Strain.
Micrographs from Suderman et al. Three-Dimensional Human Bronchial-Tracheal Epithelial Tissue-Like Assemblies (TLAs) as Hosts Fro Severe Acute Respiratory Syndrome (SARS)-CoV Infection. 2006. NASA/TP-2006-213723.)
The 3-D heterogeneous models are bioengineered to the unique specifications of the investigator using normal (non-transformed) parental cells demonstrate spontaneous recapitulation to the desired organoid tissue.
The 3-D cellular tissue models can be bioengineered on a variety of synthetic support matrices in 6-, 12- or 24- well sterile tissue culture plates.
This bioengineered format support rapid throughput analysis of therapeutic and/or prophylactic potential and cellular toxicity.
ll cells utilized for the 3-D models are early passage number acquired from a recognized commercial source, characterized by a certificate of virus and bacteria pathogen-free.
Depending on individual cell replication characteristics, the initial 3-D cell culture organoids can be available for pathogenic agent exposure and analytics within three months and subsequent 3-D models on a bi-weekly .basis for the duration of the investigation.
Cell tissue-like aggregates can be documented by light, fluorescent and confocal microscopy and/or environmental scanning (E-SEM) and transmission electron (TEM) microscopy. Media can be sampled for progeny virus, cell-pathogen derived metabolites and cellular tissue for genomic and transcriptase analytics.