Product Name: Alpha-Glia Expansion Medium
Catalog Number: AGEM-001
*Caution: The handling of human derived products has potential to be biologically. All Cell strains tested negative for HIV, HBV, and HCV
DNA in diagnostic tests. Proper precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety glasses,
etc.) when handling these materials. We recommend following the universal procedures for handling products of human origin as the minimum
precaution against contamination.
Microglial cells are the dominant antigen presenting cells in the central nervous system. Under
resting conditions, the expression of the molecular complex for presenting antigen, the major
histocompatibility complex II (MHCII) and co-stimulatory molecules such as CD80, CD86 and
CD40 is below detection. Upon injury the molecules are highly upregulated and the expression
of this complex is essential for interacting with T lymphocytes. This upregulation has been
described in a number of pathologies and is well studied in Multiple Sclerosis. Microglial cells
phagocytose myelin, degrade it and present peptides of the myelin proteins as antigens. By
releasing cytokines such as CCl2 microglial cells are important for recruiting leucocytes into the
CNS. Microglia interact with infiltrating T lymphocytes and, thus, mediate the immune response
in the brain. They have the capacity to stimulate proliferation of both TH1- and TH2-CD4
positive T cells.
Alpha-Glia Expansion Medium is a complete medium designed to promote optimal growth of
normal human brain-derived microglia in vitro. It is a sterile, liquid medium which contains:
• Essential and non-essential amino acids
• Organic and inorganic compounds
• Growth factors
• Trace minerals
• Fetal bovine serum (5%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with
an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively)
to provide a defined and optimally balanced nutritional environment that selectively promotes
growth of normal human microglia in vitro.
Alpha-Glia Expansion Medium consists of 445 ml of basal medium, 25 ml of fetal bovine serum
25 ml of microglia growth supplement and 5 ml of penicillin/streptomycin solution.
Shipping Condition: Ambient temperature (Blue ice, seasonally)
Storage condition: Alpha-Glia Expansion Medium is stored at 4°C in the dark. A change in
color or appearance of precipitate may indicate deterioration.
Shelf Life: Alpha-Glia Expansion Medium is stable for at least 2 months from the date of
receiving under proper storage condition
Use of Alpha-Glia Expansion Medium for culturing human Microglial cells:
1)-Take the amount of Alpha-Glia Expansion Medium needed and warm to 37°C in a water
bath or incubator
2)-Avoid frequent temperature change to the entire bottle of medium.
3)-Then Alpha-Glia Expansion Medium are stable for at least 1 month stored at 4°C.
4)-We recommend changing medium every 2 days for regular cell culture.