Product Name: Ames Medium w/L-Glutamine
Catalog No: PG012 Alphabioregen
Product Format: Liquid Ames Medium
Stored at 4°C w/L-Glutamine
General Information: The retina is an important in vitro model for the central nervous
system. It is more readily accessible than most nervous tissue and is strong enough to
remain intact during manipulations. The retina of the rabbit commonly contains no
penetrating blood vessels. It is nurtured by diffusion from capillary networks on either
side. For this reason, the tissue will survive and functions without circulating blood, as
long as it is bathed in a medium that closely resembles in vivo conditions.
Ames’ medium was formulated to support retinal tissue in relatively short-term culture.
Rabbit retina has been incubated in Ames’ medium for over 2 days with its metabolism
and electrical responses to light stimuli well maintained. This mixture is a medium of
choice for maintaining central nervous system tissue in vitro.
The medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and
supplements Please note that the basal medium has to be stored at 4°C up to 8°C but
never should be frozen. The supplement has to be stored at -20°C. Protect from light for
a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to touch
the tube or bottle interior threads with your fingers. Please avoid long term exposure to
light because some components are light sensitive. Also do not warm up the medium over
37°C. All this protects your medium against contamination or degradation of essential