Anti – Bcl – 2 Antibody (Monoclonal)

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Description

Product Name Anti-Bcl-2 antibody (monoclonal)
Description Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2
(BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
No cross reactivity with other proteins.
Host Mouse
Target Species Human
Application WB,HC-P, IHC-F, ICC,
Recommended Detection Systems
We recommends Enhanced Chemiluminescent Kit with anti-Mouse
IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse
IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased. Contact us for more information
Immunogen Recombinant full-length BCL2
Specificity No cross reactivity with other proteins
Pack Size 100?g/vial
Catalog Number Ab1004

Background:
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas
whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes
or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18)
translocation expressed detectable levels of BCL2 protein, regardless of whether the
breakpoint was located in or at a distance from the BCL2 gene. Overexpression of
BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
Product Properties:
Clonality Monoclonal
Clone Number Bcl-2-100
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield a concentration of 100ug/ml.
Storage At -20?C for one year. After reconstitution, at 4?C for one month. It can also be aliquoted and stored frozen at -20?C for a longer time. Avoid repeated freezing and thawing.
Purification Ascites