Product Name Anti-CDC6 Antibody (Monoclonal, DCS-180)
Description Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivitywith other proteins.
Target Species Human
Recommended Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG(EK1001) for Western blot.
Immunogen Recombinant human Cdc6.
Specificity No cross reactivity with other proteins
Pack Size 100?g/vial
Catalog # Ab1019
Background for Cell division control protein 6 homolog
In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F
proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.