Product Name : Anti-COL18A1 Rabbit Polyclonal Antibody
Endostatin, a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
ELISA:<br>Direct:<br>To detect hEndostatin by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 – 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEndostatin.<br><br>Sandwich<br>o detect hEndostatin by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 – 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Endostatin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEndostatin. <br><br>Western Blot:<br>To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
|Antigen name||collagen, type XVIII, alpha 1|
|Immunogen||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEndostatin (human Endostatin). Human Endostatin specific antibody was purified by affinity chromatography and then biotinylated.|