Anti-COL1A1 Rabbit Polyclonal Antibody

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SKU: coll-0174 Category:

Description

Product Name : Anti-COL1A1 Rabbit Polyclonal Antibody

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce ‘type’ specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Collagen I antibody can be used in ELISA, and immunohistochemistry starting at 1:50.
0.125 M sodium borate, 0.075 M NaCl, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Antigen symbolCOL1A1
Antigen namecollagen, type I, alpha 1
Antigen synonymsCOL1A1
Antibody typePrimary
ClonalityPolyclonal
ConjugationUnconjugated
ReactivityHuman,Bovine,Rat,Mouse
HostRabbit
Gene IDP02452
Western blotYes
ELISAYes
ImmunoChemistryYes
ImmunoPrecipitationYes
ImmunogenCollagen I antibody was raised against Collagen Type I from human and bovine placenta (Human).
FormLiquid
PurificationImmunoaffinity Chromatography
Concentration1 mg/mL
Storage buffer0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Storage temperatureShort term: 4 °C. Long term: Store at –20 °C. Avoid freeze-thaw cycles.
Shipping temperature4 °C
Tested applicationsImmunoaffinity Chromatography