Anti-COL2 Rabbit Polyclonal Antibody

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SKU: coll-0007 Category: Tag:

Description

Product Name: Anti-COL2 Rabbit Polyclonal Antibody.

IHC (Frozen): 1/200 – 1/1000. ELISA: 1/10000 – 1/50000. WB: 1/5000 – 1/10000. Optimal titers for other applications should be determined by the researcher. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

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Antigen symbolCOL2
Antigen nameCollagen II
Antigen synonymsCOL2A1,collagen alpha-1(II) chain, Type II Collagen,alpha-1 type II collagen, Collagen 2,chondrocalcin,cartilage collagen,alpha-1 polypeptide, collagen II
Antibody typePrimary
ClonalityPolyclonal
ConjugationBiotin
ReactivityBovine
HostRabbit
Gene ID1280
IsotypeIgG
Western blotYes
ELISAYes
ImmunochemistryYes
ImmunoprecipitationYes
ImmunogenCollagen Type II from adult human knee cartilage and bovine nasal cartilage.
PurificationPurified by antigen-affinity chromatography.
Concentration1.0 mg/ml
Storage buffer0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)
Storage temperatureFor extended storage aliquot contents and freeze at –20 °C or below. Avoid cycles of freezing and thawing.
Tested applicationsELISA, IHC-Fr, IP, WB