Product Name : Anti-COL2 Rabbit Polyclonal Antibody
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “”Glycine-X-Y”” triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
Recommended Starting Dilutions:For WB (non-denaturing, non-dissociating): 1:5,000-1:10,000For Indirect ELISA (against 1.0?g collagen II): 1:4,000-1:8,000 For IHC (frozen sections): 1:50-1:200. Optimal titers for other applications should be determined by the researcher. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used, as well as formalin fixed tissues in IHC.
|Antigen name||Collagen II|
|Antigen synonyms||COL2A1,collagen alpha-1(II) chain,Type II Collagen,alpha-1 type II collagen,Collagen 2,chondrocalcin,cartilage collagen,alpha-1 polypeptide,collagen II|
|Immunogen||Collagen Type II from human knee cartilage and bovine nasal cartilage.|
|Purification||Purified by antigen-affinity chromatography.|
|Storage buffer||0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide|
|Storage temperature||For extended storage, aliquot contents and freeze at –20 °C or below. Avoid cycles of freezing and thawing.|
|Tested applications||ELISA, IHC-Fr, IP, WB|