Product Name : Anti-COL4A1 Rabbit Polyclonal Antibody
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce ‘type’ specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with type I, II, III, V, or VI collagens.
Collagen IV antibody can be used in immunohistochemistry starting at 20 ?g/mL.
0.125 M sodium borate, 0.075 M NaCl, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
|Antigen name||collagen, type IV, alpha 1|
|Immunogen||Collagen IV antibody was raised against amino acids from the C-Terminus of Collagen Type IV from human and bovine placenta (Human).|
|Storage buffer||0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.|
|Shipping temperature||4 °C|
|Tested applications||Immunoaffinity Chromatography|