Anti-Collagen type 1 Rabbit Polyclonal Antibody

$USD2,014

$USD100.75 Cashback
SKU: coll-00148 Category:

Description

Product Name : Anti-Collagen type 1 Rabbit Polyclonal Antibody

The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic ¾ and ¼ fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen ¾ fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.
Immunocytochemistry: (2.5-10ug/ml). Optimal conditions must be determined individually for each application.
Antigen nameCollagen type 1
Antibody typePrimary
ClonalityPolyclonal
ConjugationUnconjugated
ReactivityHuman,Bovine,Rat,Mouse,Cat,Sheep,Dog,Hamster,Pig
HostRabbit
ImmunogenSynthetic peptide (human sequence) corresponding to the C-terminal end of the N-terminal three quarter collagen fragment (Col1 ¾).
FormLiquid
PurificationAntigen affinity purified.
Concentration250 ug/mL
Storage bufferLiquid. In PBS with 1 mg/ml BSA and 0.02% sodium azide.
Storage temperatureStable for at least 1 year after receipt when stored at –20 °C. Stable for at least 6 months after receipt when stored at –20 °C.
Shipping temperature4 °C
Tested applicationsImmunocytochemistry