Anti-GP6 Rabbit Polyclonal Antibody

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SKU: coll-0266 Category:

Description

Product Name : Anti-GP6 Rabbit Polyclonal Antibody

Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Collagen receptor involved in collagen-induced platelet adhesion and activation. GP6 plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2.Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets. Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels. Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation. In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss. However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke (Jandrot-Perrus et al., 2000 [PubMed 10961879]).[supplied by OMIM].

 

GP6 antibody can be used for detection of GP6 by ELISA at 1:1562500. GP6 antibody can be used for detection of GP6 by western blot at 1 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Antigen symbolGP6
Antigen nameglycoprotein VI (platelet)
Antigen synonymsGP6
Antibody typePrimary
ClonalityPolyclonal
ConjugationUnconjugated
ReactivityHuman
HostRabbit
Gene IDQ9HCN6
Western blotYes
ELISAYes
ImmunogenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GP6.
FormLiquid
Molecular weight37 kDa
PurificationAntibody is purified by peptide affinity chromatography method.
Storage bufferPurified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Storage temperatureFor short periods of storage (days) store at 4 °C. For longer periods of storage, store GP6 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
Shipping temperature4 °C