Dulbecco’s MEM (DMEM) Ham’s F-12

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Description

Product Name: Dulbecco’s MEM (DMEM) Ham’s F-12
Catalog No: PG026
Product Format: Liquid
Stored at 4°C

General Information: Dulbecco’s MEM (DMEM) Ham’s F-12 w/o HEPES, Glucose, Amino
Acids, Linoleic Acid, Lipoic Acid, is the most widely used modification of BME. It contains
a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and
certain essential trace elements were added. The bicarbonate concentration was increased.
The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with
4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed
for the culture of mice embryonic cells. Today, it finds a broad application of serum free
culture of normal and transformed mouse and chicken cells.
Ham’s Nutrient Mixtures were originally developed to support growth of several clones of
Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were
formulated for use with or without serum supplementation, depending on the cell type
being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat
prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells. The
medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and
supplements Please note that the basal medium has to be stored at 4°C up to 8°C but
never should be frozen. The supplement has to be stored at -20°C. Protect from light for
a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to touch
the tube or bottle interior threads with your fingers. Please avoid long term exposure to
light because some components are light sensitive. Also do not warm up the medium over
37°C. All this protects your medium against contamination or degradation of essential
components.