Eagle’s Basal Medium (BME)




Product Name: Eagle’s Basal Medium (BME)
Catalog No: PG014
Product Format: Liquid
Stored at 4°C

General Information: Eagle’s Basal Medium (BME) was developed by Harry Eagle. BME
forrmula was developed as a result of numerous studies carried out in the late 1950’s to
determine the essential nutritional requirements and other factors critical to the growth of
cells in culture. Historically, BME has been used in studies conducted to measure the
growth response of normall (WI-38) and transformed (mouse and HeLa) cells in monolayer
growth. It is one of the most widely used of all synthetic cell culture media.
The medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and
supplements Please note that the basal medium has to be stored at 4°C up to 8°C but
never should be frozen. The supplement has to be stored at -20°C. Protect from light for a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to touch
the tube or bottle interior threads with your fingers. Please avoid long term exposure to
light because some components are light sensitive. Also do not warm up the medium over
37°C. All this protects your medium against contamination or degradation of essential