Human Primary Neurons.
Human Primary Neurons are initiated by digestion of minced brain cortical tissue with collagenase. Human neurons are separated from the mixture of cell populations and offered at passage 3 in a frozen vial. HNCs Growth Medium (containing 10% serum) and growth supplements, cat# RB-PHN003is recommended for cell culture. These cells have an average population doubling capacity of 8 when cultured following the detailed protocol.
The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain . Despite great variability in size and shape, all neurons share common morphologic features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The human brain contains about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons .
HN from Regeneration Biology” Pharmaceutical, Research Labs, Products & Services are isolated from human brain. HN are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HN are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and ?-tubulin III. HN are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN are guaranteed to further culture under the conditions provided by Regeneration Biology” Pharmaceutical, Research Labs, Products & Services.; however, HN are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
|Country of Manufacture||United States|
|Size/Quantity||1 x 10^6 cells/vial|
|Product Use||This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.|
|Storage||Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.|
|Shipping Info||Dry ice.|
|References||1] Parent, A. (1996) Neurons in Carpenter’s Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.
 Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.