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Name of Products
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Human Pulmonary
Artery Smooth Muscle Cells
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Catalog No
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UBP- 0101
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Product Format
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Frozen Vial
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Cell Number
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> 5×10[5]
cells/vial
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GENERAL INFORMATION
HPASMC has been used to show that IL-22
promotes the growth of pulmonary vascular SMCs via a signaling mechanism that
involves NADPH oxidase-dependent oxidation (Bansal, 2013). Inducers of
pulmonary hypertension, characterized by thickened pulmonary arterial walls,
activate expression of anti-apoptotic Bcl-xL gene via binding of GATA-4 to
its promoter; the activation can be suppressed by targeting gata4 gene
transcription (Suzuki, 2007).
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PRODUCT USAGE The cells are offered for Research Use Only.
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SHIPPING Frozen Vials in a Dry Ice Package.
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HANDLING OF ARRIVING
CELLS When you receive the dry ice
package with cells in frozen vials, transfer the frozen vials of cells into a
-80C freezer for short period storage or a liquid nitrogen tank for long-
term storage.
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PROTOCOLS FOR
THAWING THE CELLS AND SUBCULTURE
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A)
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Pre-coating of T25
flasks- Add 2ml each Universal Coating Solution (UBP-01) into a T25 flask to
cover the whole surface of the flask, 5 mins later, dispose the excessive
coating solution by aspiration and the flask is ready to be used (although
solution containing other extracellular matrix, i.e. gelatin, collagen, and
fibronectin, can be used, make sure to optimize the conditions in advance).
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B)
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Thaw the frozen cell
vial in a 37C water bath first, and then transfer the cells into the
pre-coated T25 flask with 10ml of Full medium, cells usually become confluent
with 5-7 days.
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C)
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To passage the
cells, rinse the cells in a T25 flask with 5ml HBSS (RT) twice; then add 2ml Universal
Detachment Solution (RT) (UBP-23) into one T25 flask; gently dispose the
excessive Universal Detachment Solution within 20 seconds by aspiration.
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D)
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Leave the T25 flask
with the cells at RT or 37C for 1 min (most cells usually will detach from
the surface within 1-2 mins; or monitor the cells under a microscope until
most of cells become rounded up, and then gently tap the flask against the
bench surface, and the cells will move on the surface of the flask when
monitoring under microscope.
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E)
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Add 5ml Universal
Neutralization Buffer and spin down the cells with 800g centrifugation for 5
mins.
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G)
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Re-suspend the cell
pellet with 10 or 15ml Full medium and transfer 5 ml each into 2 or 3
pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio).
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H)
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Change medium every
2 or 3days and the cells usually become confluent within 7 days (when split
at a 1/3 ratio).
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