Human Recombinant Endothelial cell adhesion molecule

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Description

Product Name : Human Recombinant Endothelial cell adhesion molecule

Endothelial Cell Adhesion Molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein member of the JAM family of immunoglobulin superfamily molecules. The 390 amino acid Human ESAM contains a 216 amino acid extracellular domain (ECD) with a V-type and a C2-type immunoglobulin (Ig) domain. ESAM is specifically expressed at endothelial tight junctions and on activated platelets and performs homophilic adhesion activity. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. In addition, ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. ESAM-deficient mice were described with lowered angiogenic potential, and accordingly, overexpression of ESAM is closely associated with certain tumor growth and metastasis. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes. The ECD of human and mouse ESAM share 69% amino acid identity.
  • Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only
Protein/peptide nameEndothelial cell adhesion molecule
Protein synonymsESAM,Endothelial Cell-Selective Adhesion Molecule
Protein/peptide typeRecombinant
SpeciesHuman
SourceCells
SequenceGln30-Ala247
Endotoxin content<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Purity> 95% as determined by reducing SDS-PAGE
FormulationLyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular weight24.79 kD
Storage conditionsLyophilized protein should be stored at ?20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at ?20 °C for 3 months.
Tag sequenceC-6 His tag
Tested applicationsBioassay