Human Recombinant FGF1

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SKU: hec-054 Category:

Description

Product Name : Human Recombinant FGF1

FGF acidic, also known as ECGF, FGF-1and HBGF-1, is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal, ectodermal, and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta, exported to the cytosol, dephosphorylated, and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.

 

This recombinant protein can be used for biological assays. For research use only.
Protein/peptide nameFGF1
Protein synonymsFGF acidic,FGF-1,Acidic Fibroblast Growth Factor,ECGFHeparin-Binding Growth Factor 1,Fibroblast Growth Factor 1,Endothelial Cell Growth Factor,aFGF,FGF1,FGFA,HBGF-1
Protein/peptide typeRecombinant
SpeciesHuman
SourceE. coli
SequencePhe16-Asp155
Endotoxin content<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Purity> 95% as determined by reducing SDS-PAGE
FormulationLyophilized from a 0.2 um filtered solution of 20mM Tris, 400mM NaCl, 1mM DTT, pH 8.0 . Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular weight15.9 kD
Storage conditionsLyophilized protein should be stored at ?20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at ?20 °C for 3 months.
Tested applicationsBioassay