Leibovitz L-15 Medium w/L-Glutamine, HEPES, Tris, Low Sodium




Product Name: Leibovitz L-15 Medium w/L-Glutamine, HEPES, Tris, Low Sodium
Catalog No: PG059
Product Format: Liquid
Storage: 4°C

General Information: The most commonly used buffering system for mammalian cell
cultures is a bicarbonate/CO2 system, which requires CO2 regulators and incubators to
supply a constant level of CO2. As a replacement, Leibovitz developed a bicarbonate-free
medium, L15, with relatively high levels of certain amino acids in the free base form.
The medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and supplements
Please note that the basal medium has to be stored at 4°C up to 8°C but never should be
frozen. The supplement has to be stored at -20°C. Protect from light for a longer time
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to
touch the tube or bottle interior threads with your fingers. Please avoid long term
exposure to light because some components are light sensitive. Also do not warm up the
medium over 37°C. All this protects your medium against contamination or degradation
of essential components.