Product Name: MCDB 105 Diploid Fibroblast-like Cell Medium
Catalog No: PG109
Product Format: Liquid
Basal Medium MCDB 105 is specifically formulated for the cultivation
of diploid fibroblast-like cells. MCDB 105 and 110 are modifications of MCDB 104
medium. These modifications are optimized for long-term survival and rapid clonal growth
of human diploid fibroblast-like cells (WI-38, MRC-5, IMR-90) and of low-passage human
foreskin fibroblasts using FBSP or hormone and growth factor supplements. Supplement
with growth factors or fetal calf serum (0.2-2.0% dialyzed).
The medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and supplements
Please note that the basal medium has to be stored at 4°C up to 8°C but never should be
frozen. The supplement has to be stored at -20°C. Protect from light for a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to
touch the tube or bottle interior threads with your fingers. Please avoid long term
exposure to light because some components are light sensitive. Also do not warm up the
medium over 37°C. All this protects your medium against contamination or degradation
of essential components.