Product Name: MEM Alpha Modified w/ L-Glutamine, Ribo, Deoxyribonucleosides
Catalog No: PG066
Product Format: Liquid
General Information: Minimum Essential Medium (MEM), developed by Harry Eagle, is one
of the most widely used of all synthetic cell culture media. Early attempts to cultivate
normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had
specific nutritional requirements that could not be met by Eagle’s Basal Medium (BME).
Subsequent studies using these and other cells in culture indicated that additions to BME
could be made to aid growth of a wider variety of fastidious cells. MEM, which
incorporates these modifications, includes higher concentrations of amino acids so that the
medium more closely approximates the protein composition of mammalian cells. MEM has
been used for cultivation of a wide variety of cells grown in mono-layers.
The medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and supplements
Please note that the basal medium has to be stored at 4°C up to 8°C but never should be
frozen. The supplement has to be stored at -20°C. Protect from light for a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to
touch the tube or bottle interior threads with your fingers. Please avoid long term
exposure to light because some components are light sensitive. Also do not warm up the
medium over 37°C. All this protects your medium against contamination or degradation
of essential components.