Mouse Recombinant Collagen alpha-1(III) chain


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Product Name: Mouse Recombinant Collagen alpha-1(III) chain.

Collagen alpha-1(III) chain?Col3a1? is a secreted protein and belongs to the fibrillar collagen family. It contains 1 fibrillar collagen NC1 domain and 1 VWFC domain. Collagen alpha-1(III) chain is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I collagen. The COL3A1 gene produces the components of type III collagen, called pro-alpha1(III) chains. Three copies of this chain combine to make a molecule of type III procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell to remove extra protein segments from their ends. Once these molecules are processed, the collagen molecules arrange themselves into long, thin fibrils. Within these fibrils, the individual collagen molecules are cross-linked to one another. These cross-links result in the formation of very strong mature type III collagen fibrils, which are found in the spaces around cells.
Protein/peptide nameCollagen alpha-1(III) chain
Protein synonymsCol3a1,Collagen alpha-1(III) chain
Protein/peptide typeRecombinant
Endotoxin content<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Purity> 95% as determined by reducing SDS-PAGE
FormulationSupplied as a 0.2 um filtered solution of 20mM HAc-NaAc,150mM NaCl, pH4.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular weight96.6 kD
Storage conditionsStore at ?20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Tag sequenceC-6 His tag
Tested applicationsBioassay
Molecular weight96.6 kD
This recombinant protein can be used for biological assays. For research use only.