Principle: Real-time fluorescence PCR technology and hydrolysis probe technology is used to achieve qualitative detection of ORF1ab and N genes at the nucleic acid level of one or more pathogens in the same reaction tube. The instrument software system automatically draws real-time application curves to achieve instant results Judge.
Detection time: 75-110 minutes
Test samples: whole blood, plasma, serum, throat swabs
Advantages: high sensitivity and strong specificity
Disadvantages: low temperature transportation, long detection time, and high requirements for experimental conditions
Reagent composition: RT-PCR reaction solution, enzyme mixed solution, new coronavirus (2019) reaction solution, positive control, negative control.
Minimum detection limit: 1 × 10 3 copies / ml;
Linear detection range: 2 × 10 3 ~ 1 × 10 8 copies / ml
Specificity: other pathogens with the same infection site or similar and common symptoms (coronavirus 229E , coronavirus OC43 , coronavirus HKU1 , coronavirus NL63 , influenza A virus, influenza B virus, parainfluenza virus , Adenovirus, respiratory syncytial virus, metapneumovirus, boca virus, rhinovirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Legionella, Staphylococcus aureus, Mycobacterium tuberculosis, influenza Haemophilus, Acinetobacter baumannii) and human leukocytes have no cross-reactive total nucleic acids. Precision: The coefficient of variation of the detection precision reference is less than 5%.