Total Collagen Assay Kit


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Product Name: Total Collagen Assay Kit (Colorimetric)

A simple, sensitive & easy colorimetric assay to quantitate total collagen levels.
  • Measurement of collagen in various sample types
  • Can detect as low as 0.5 µg of collagen
  • Based on the acid hydrolysis of samples to form hydrolysates
Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals. The triple-helical structure of collagen is quite unique: it consists of a repeating pattern of a basic trimer: Glycine-Proline-Hydroxyproline. In cells, collagens are secreted as procollagens and these chains are transported into the Endoplasmic Reticulum, where, numerous post-translational modifications lead to the formation of a triple helix with disulfide bonds. Excessive production of collagen is linked to pathological conditions including liver cirrhosis, lung fibrosis, and tumor growth. Regeneration Biology LTD Collagen Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore (Abs 560 nm). The assay is simple, sensitive and specific for collagen and can detect as low as 0.5 µg of collagen in a variety of samples such as tissue homogenates, biological fluids and purified proteins.
Species reactivity Mammalian tissues, protein/peptide hydrolysates, serum, urine
Shelf life 12 months
Sample type Mammalian tissues
Protein/peptide hydrolysates
Detection method Absorbance (OD = 560 nm)
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